Eliciting broadly neutralizing antibodies against HIV-1 that target gp41 MPER
نویسندگان
چکیده
Methods We generated a mini-protein that is structurally rigid, yet efficiently recognized by 2F5, 4E10 and Z13e1. It contains the C-terminal 54 a.a. of gp41 ectodomain (gp41-54Q), which includes the HR2 and the MPER. A 6xHis tag at the C-terminus was used to attach gp41-54Q to Zn-chitosan, which served as an antigen carrier/adjuvant. Rabbits were immunized subcutaneously, 4 times, using two different schedules (wks 0, 4, 9 and 16 vs. 0, 8, 16 and 24). A total of 9 animals were immunized with gp41-54Q in 3 independent experiments. Antibody responses were evaluated by ELISA and in neutralization assays using both TZM-bl and A3R5.7 cells.
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Induction of HIV Neutralizing Antibodies against the MPER of the HIV Envelope Protein by HA/gp41 Chimeric Protein-Based DNA and VLP Vaccines
Several conserved neutralizing epitopes have been identified in the HIV Env protein and among these, the MPER of gp41 has received great attention and is widely recognized as a promising target. However, little success has been achieved in eliciting MPER-specific HIV neutralizing antibodies by a number of different vaccine strategies. We investigated the ability of HA/gp41 chimeric protein-base...
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